Publications

J.R. Hepler Publications - PubMed 

 

Selected publications from the Hepler Lab with abstract links:

Brown, N.E., Blumer, J.B., and Hepler, J.R. (2015) Bioluminescence Resonance Energy Transfer to Detect Protein-Protein Interactions in Live Cells. In: Protein-Protein Interactions: Methods and Applications, Methods in Molecular Biology , 1278:457-65.Abstract

Brown, N.E., Goswami, D., Branch, M.R., Ramineni, S., Ortlund, E.A., Griffin, P.R. and Hepler, J.R. (2015) Integration of G protein alpha (Gα) Signaling by the Regulator of G protein Signaling 14 (RGS14). J. Biol. Chem ., Apr 3;290(14):9037-49. Abstract

Evans, P.R., Dudek, S. M., and Hepler, J.R. (2015) Regulator of G Protein Signaling 14: A Molecular Break on Synaptic Plasticity Linked to Learning and Memory. Prog Mol Biol Transl Sci., in press.

Brown, N.E., Lambert, N.A and Hepler, J.R. (2016) RGS14 regulates the lifetime of Gα-GTP signaling but does not prolong Gβγ signaling following receptor activation in live cells. Pharmacol Res Perspectives.   Aug 18;4(5):e00249
https://www.ncbi.nlm.nih.gov/pubmed/27713821

Gerber, K.J., Squires, K.E. and Hepler, J.R. (2016) Roles for Regulator of G protein Signaling (RGS)Proteins in Synaptic Signaling and Plasticity.  Molecular Pharmacology, 89:273-86.
https://www.ncbi.nlm.nih.gov/pubmed/26655302

Ghil, SH, McCoy, K.L. and Hepler, J.R. (2014) RGS2 and RGS4 form distinct G protein-dependent complexes with protease activated recepetor-1 (PAR1) in live cells. PLoS ONE, 9(4):e95355. Doi:10.1371. Abstract

Hepler, J.R. (2014) G protein coupled receptor signaling complexes in live cells. Cellular Logistic, 4(1):e29392. Abstract

Evans, P.R., Lee, S.E., Smith, Y., and Hepler, J.R. (2014) Postnatal Developmental Expression of Regulator of G Protein Signaling 14 (RGS14) in the Mouse Brain. J. Comp. Neurol, 522 (1): 186-203. (DOI:10.1002/cne.23395 (online pub, Nov 26, 2013). Abstract

Vellano, C.P., Brown, N.E., Blumer, J.B. and Hepler, J.R. (2013) Assembly and function of the regulator of G protein signaling 14 (RGS14):H-Ras complex is regulated by Gαi1 and a Gi-linked GPCR. J. Biol. Chem., 288(5):3620-31. doi: 10.1074/jbc.M112.440057. Epub 2012 Dec 17. PMID: 23250758 Abstract

Zhao, P., Nunn, C., Ramineni, S., Hepler, J.R. and Chidiac, P. (2012) The Ras-binding domain of RGS14 regulates its functional interactions with heterotrimeric G proteins. J. Cell. Biochem., 114(6): 1414-1423. doi: 10.1002/jcb.24483. Abstract

McCoy, K.L., Gyoneva, S., Vellano, C.P., Smrcka A.V., Traynelis, S.F. and Hepler, J.R. (2012) Protease-Activated Receptor 1 (PAR1) coupling to Gq/11 but not to Gi/o or G12/13 is mediated by discrete amino acids within the receptor second intracellular loop. Cellular Signalling, 24(6):1351-60. Abstract

Vellano, C.P., Maher, E.M., Hepler, J.R. and Blumer, J.B. (2011) G protein coupled receptors and Ric-8A both regulate the Regulator of G protein Signaling 14 (RGS14):Gαi1 complex in living cells. J. Biol. Chem. 286(44): 38659-3866. Abstract

Vellano, C.P, Lee, S.E., Dudek, S.M. and Hepler, J.R. (2011) RGS14 at the interface of hippocampal signalling and synaptic plasticity. Trends in Pharmacological Sciences32(11):666-674. Abstract

Vellano CP, Shu FJ, Ramineni S, Yates CK, Tall GG, Hepler JR (2011) Activation of the regulator of G protein signaling 14-Gαi1-GDP signaling complex is regulated by resistance to inhibitors of cholinesterase-8A. Biochemistry 50(5):752-62. Abstract


Lee, S.E., Simmons, S., Heldt, S.A., Schroeder, J.A, Zhao, M., Cowan, D.P., Vellano, C.P., Feng, Y., Weinshenker, D., Sweatt, D.L. Ressler, K.J., Dudek, S.M., and Hepler, J.R. (2010) RGS14 is a natural suppressor of both synaptic plasticity in CA2 neurons and hippocampal-based learning and memory. Proc. Natl. Acad. Sci, USA 107(39):16994-8.Abstract

Jernigan KK, Cselenyi CS, Thorne CA, Hanson AJ, Tahinci E, Hajicek N, Oldham WM, Lee LA, Hamm HE, Hepler JR, Kozasa T, Linder ME, Lee E (2010) Gbetagamma activates GSK3 to promote LRP6-mediated beta-catenin transcriptional activity. Science Signaling, 3(121):ra37. Abstract

McCoy, K.L., Traynelis, S.F. and Hepler, J.R. (2010) PAR1 and PAR2 couple to overlapping and distinct sets of G proteins and linked signaling pathways to regulate cell physiology.Molecular Pharmacology 77(6):1005-15. Abstract

Shu, F.J., Ramineni, S and Hepler, J.R. (2010) RGS14 is a multifunctional scaffold that integrates G protein and H-Ras/Raf/MAP kinase signaling pathways. Cellular Signalling22(3):366-76. PMID: 19878719. Abstract


McCoy, K.L., and Hepler, J.R. (2009) RGS proteins as central components of the G protein coupled receptor signaling complex.   Progress in Mol. Biol. Translational. Sci, 86; 49-74.Abstract


Pedone H. K., Bernstein, L.S., Linder, M.E., and Hepler, J.R. (2008) Measurement of protein palmitoylation. The Protein Protocols Handbook, Third Edition, Humana Press, (John Walker, Editor), pgs. 1613-1626. PDF file


Mannaioni, G., Goldshmidt, A., Hamill, C., Yuan, H., Pedone, K. H., Junge, C. E., Lee, C.J., Yepes, M., Hepler, J.R. , and Traynelis, S.F. (2008) Plasmin potentiates synaptic NMDA receptor function in rat hippocampal neurons through activation of PAR1. J. Biol. Chem.,  283(29): 20600-20611. Abstract


Pedone, K.H. and Hepler, J.R. (2007) The importance of amino terminal polycysteine and polybasic sequences  in  determining  G14α and G16α palmitoylation, plasma membrane targeting and signaling functions.  J. Biol. Chem.. 282: 25199-25212. Abstract


Gu, S., He, J., Ho, W.T., Ramineni, S., Thal D.M., Natesh, R., Tesmer, J.J.G., Hepler, J.R. and Heximer, S.P. (2007). Unique hydrophobic extension of the RGS2 amphipathic helix domain imparts increased plasma membrane binding and function relative to other RGS R4/B subfamily members. J. Biol. Chem., 282: 33064-75. Abstract


Shu F-J, Ramenini,S., Amyot, W.M., and Hepler, J.R.  (2007) Selective interactions between Giα1 and Giα3 and the GoLoco/GPR domain of RGS14 influence its dynamic subcellular localization. Cellular Signalling, 17: 383-389. Abstract


Neitzel, K.L. and Hepler, J.R. (2006) Cellular mechanisms that determine selective regulation of G protein coupled receptor signaling by RGS proteins. Seminars in Developmental and Cellular Biology, 17: 383-389. Abstract


Roy, A.A., Baragli, A., Bernstein, L.S., Hepler, J.R., Hebert, T.E., and Chidiac, P. (2006) RGS2 interacts with adenylyl cyclase in living cells. Cellular Signalling., 18:336-48.Abstract


Hubbard, K.B and Hepler, J.R (2006) Cell signaling diversity of the Gqα family of heterotrimeric G proteins. Cellular Signalling, 18:135-50. Abstract


Hepler, JR. (2005) R7BP: A surprising new link between G proteins, RGS proteins and nuclear signaling in brain. Science STKE, 294, pe38. Abstract


Nicole, O., Sorensen, S.D., Sastre, A. Hepler, J.R.. Brat, D., McKeon, R.,  and Traynelis, S.F. (2005)  Activation of protease activated receptor-1 (PAR-1) contributes to glial scar formation after brain injury.  J. Neuroscience, 25, 4319-4329. Abstract


Hague, C., Bernstein, L.S., Ramenini,S., Chen, Z.J., Minneman, K.P. and Hepler, J.R. (2005) Selective inhibition of α1A-adrenergic receptor signaling by RGS2 association with the receptor third intracellular loop. J .Biol Chem., 280, 27289-27295. Abstract


Peavy, R.B., Hubbard, K.B., Lau, A.G., Fields, B., Lee, T.T., Gernert, K., Murphy, T. J., and Hepler, J.R. (2005) Differential effects of Gqα, G14α and G15α on vascular smooth muscle cell survival and gene expression profiles. Molecular Pharmacology, 67:2102-14. Abstract


Hepler, J.R., Cladman, W. Ramenini, S. Hollinger, S. and Chidiac, P. (2005) Novel activity of RGS14 on Goα and Giα nucleotide binding and hydrolysis independent of its RGS domain and GDI activity.  Biochemistry, 44, 5495-5502. Abstract


Bernstein, L.S., Ramineni, S., Chris Hague, Wendy Cladman, Peter Chidiac, Levey, A.I. and Hepler, J.R.  (2004) RGS2 binds directly and selectively to the M1 muscarinic cholinergic receptor third intracellular loop to modulate Gq/11α signaling.J. Biol. Chem., 279, 21248-21256. Abstract


Bernstein, L.S., Linder, M.E. and Hepler, J.R. (2004)  Analysis of RGS protein palmitoylation. Meth. Mol. Biol.. 237, 195-204. Abstract


Hollinger, S. and Hepler, J.R. (2004) Methods for measuring phosphorylation of RGS proteins by G protein regulated kinases. Meth. Mol. Biol., 237, 205-219. Abstract


Junge, C.E., Lee, J.C, Hubbard, K.B., Zhang, Z., Olsen, J.J., Hepler, J.R., Brat, D.J., Traynelis, S.F. (2004) Protease activated receptor-1 (PAR-1) in human brain: localization and functional expression in astrocytes. Experimental Neurology, 188, 94-103. Abstract


Hepler, J.R. (2003) RGS protein and G protein interactions: A little help from their friends.Molecular Pharmacology., 64, 547-549. Abstract

Hollinger, S., Ramineni, S., and Hepler, J.R. (2003) Phosphorylation of RGS14 by protein kinase A modulates its activity toward Gαi, Biochemistry, 42, 811-819. Abstract

Sorensen, S.D., Nicole, O., Peavy, R.D., Montoya, L.M., Lee, J.C., Murphy, T.J., Traynelis, S.,F. and Hepler, J.R. (2003) Common signaling pathways link activation of murine PAR-1, LPA and S1P receptors to proliferation of astrocytes.Molecular Pharmacology., 64, 1199-1209. Abstract

Hollinger, S. and Hepler, J.R. (2002) Cellular roles and regulation of RGS proteins: modulators and integrators of G protein signaling Pharmacological Reviews, 54(3), 527-559. Abstract

Hollinger, S., Taylor, J.B.,  Hoag-Goldman, E.M., and Hepler, J.R. (2001) RGS14 is a  bifunctional regulator of Gαi/o activity that exists in multiple populations in brain. 
J. Neurochem., 79, 941-949. Abstract


Cunningham, M.L., Waldo, G.L., Hollinger, S.,  Hepler, J.R. and Harden, T.K. (2001) PKC phosphorylates RGS2 and modulates its capacity for negative regulation of Gα11 signaling.J. Biol. Chem., 276, 5438-5444. Abstract

Chidiac, P., Gadd, M.E. and Hepler, J.R.  (2001) Methods for measuring RGS interactions with Gqα. Methods  Enzymol., 344(B), 686-702. Abstract


Rose, J.J., Taylor, J.B., Shi, J., Jones, P.G, Cockett, M.R. and Hepler, J.R. (2000) RGS7 is palmitoylated and exists as biochemically distinct populations. J. Neurochem., 75, 2103-2112. Abstract


Heximer, S. P., Srinivasa, S.P., Bernstein, L.S., Bernard, J.L., Linder, M.E., Hepler, J.R. and  Blumer, K.J. (1999) G protein selectivity is a determinant of RGS2 function. J. Biol. Chem., 274, 34253-34259. Abstract


Hepler, J.R. (1999) Emerging roles for RGS proteins in cell signaling. Trends Pharmacol. Sci. 20, 376-382. Abstract


Saugstad, J.A., Marino, M.J., Folk, J.A., Hepler, J.R. and Conn, P.J.  (1998)  RGS4 inhibits signaling by group I metabotropic glutamate receptors.  J. Neuroscience 18, 905-913.Abstract


Heximer, S.P., Watson, N., Linder, M.E., Blumer, K.J. and Hepler, J.R. (1997) RGS2/GOS8 is a selective inhibitor of Gqα function.  Proc. Natl. Acad. Sci. USA 94, 14389-14393.Abstract

Hepler, J.R., Berman, D.M., Gilman, A.G. and Kozasa, T. (1997) RGS4 and GAIP are GAPs for Gqα and block activation of phospholipase Cβ by GTPγS-Gqα. Proc. Natl. Acad. Sci. USA 94: 428-432. Abstract


Huang C, Hepler JR, Gilman AG, Mumby SM. (1997) Attenuation of Gi- and Gq-mediated signaling by expression of RGS4 or GAIP in mammalian cells.  Proc. Natl. Acad. Sci US, Jun 10; 94(12): 6159-63. Abstract

Huang C, Hepler JR, Chen LT, Gilman AG, Anderson RG, Mumby SM (1997) Organization of G proteins and adenylyl cyclase at the plasma membrane. Mol Biol Cell. Dec 8 (12): 2365-78. Abstract



Hepler, J.R., Biddlecome, G.H., Kleuss, C., Camp, L.A., Hofmann, S.L., Ross, E.M. and Gilman, A.G. (1996) Functional importance of the amino terminus of Gqα.  J. Biol. Chem. 271, 496-504. Abstract


Taussig, R., Tang, W.-J., Hepler, J.R. and Gilman, A.G. (1994) Distinct patterns of bidirectional regulation of mammalian adenylyl cyclases. J. Biol. Chem. 269, 6093-6100.Abstract


Hepler, J.R., Kozasa, T. and Gilman, A.G. (1994) Purification of recombinant Gqα, G11α, and G16α from Sf9 cells. Meth. Enzymol. 237, 191-212. Abstract


Kozasa, T., Hepler, J.R., Smrcka, A.V., Simon, M.I., Rhee, S.G., Sternweis, P.C. and Gilman, A.G. (1993) Purification and characterization of recombinant G16 from Sf9 cells: Activation of purified phospholipase C isozymes by G-protein subunits. Proc. Natl. Acad. Sci. USA 90, 9176-9180. Abstract


Hepler, J.R., Kozasa, T., Smrcka, A.V., Simon, M.I., Rhee, S.G., Sternweis, P.C. and Gilman, A.G. (1993) Purification from Sf9 cells and characterization of recombinant Gqα and G11α.  Activation of purified phospholipase Cβ isozymes by Gα subunits.   J. Biol. Chem. 268: 14367-14375. Abstract


Linder, M.E., Middleton, P., Hepler, J.R., Taussig, R., Gilman, A.G. and Mumby, S.M. (1993) Lipid modifications of G proteins: subunits are palmitoylated. Proc. Natl. Acad. Sci. U.S.A. 90, 3675-3679. Abstract


Hepler, J.R. and Gilman, A.G. (1992) G Proteins. Trends Biochem. Sci. 17, 383-387.Abstract


Smrcka, A.V., Hepler, J.R., Brown, K.O., Sternweis, P.C. (1991) Purified Gq regulates polyphosphoinositide-specific phospholipase C activity.  Science 251, 804-807. Abstract


Hepler, J.R., Jeffs, R.A., Huckle, W.R., Outlaw, H.E., Rhee, S.G., Earp, H.S. and Harden, T.K. (1990) Evidence that the epidermal growth factor and non-tyrosine kinase hormone receptors stimulate phosphoinositide hydrolysis by independent pathways.  Biochem. J.270, 337-344. Abstract


Hepler, J.R., Earp, H.S. and Harden, T.K. (1988) Long-term phorbol ester treatment down-regulates protein kinase C (PKC) and sensitizes the phosphoinositide signalling system to hormone and growth factor stimulation: Evidence for a role of PKC in agonist-induced desensitization. J. Biol. Chem. 263, 7610-7619. Abstract


Hepler, J.R., Nakahata, N., Lovenberg, T.W., DiGuiseppi, J., Herman, B., Earp, H.S. and Harden, T.K. (1987) Epidermal growth factor stimulates the rapid accumulation of inositol (1,4,5) trisphosphate and a rise in cytosolic calcium-mobilized from intracellular stores in A431 cells. J. Biol. Chem. 262, 2951-2956. Abstract


Hepler, J.R. and Harden, T.K. (1986) Guanine nucleotide-dependent, pertussis toxin-insensitive stimulation of inositol phosphate formation by carbachol in a membrane preparation from human astrocytoma cells. Biochem. J. 239, 141-146. Abstract

Nakahata N, Martin MW, Hughes AR, Hepler JR, Harden TK (1986) H1-Histamine receptors on human astrocytoma cells. Mol Pharmacol. 1986 Feb;29 (2): 188-95. Abstract

Evans T, Hepler JR, Masters SB, Brown JH, Harden TK. (1985) Guanine nucleotide regulation of agonist binding to muscarinic cholinergic receptors. Relation to efficacy of agonists and for stimulation of phosphoinositide breakdown and calcium mobilization.Biochem J.  Dec 15; 232(3): 751-7. Abstract